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Stephen Lamart

qPCR expert at Veolia Environnement

Chatillon, France



Stephen Lamart

  English   |   French
Centre d’analyses environnementales (CAE), St-Maurice (94) - Veolia Environnement - France

qPCR expert at the R&D laboratory of microbiology :

- Development of microorganisms detection system using DNA or RNA amplification and qPCR (TaqMan mainly) : fungi, virus, pathogen bacteria, protozoans...
- Nucleic acid extraction methods assessment from various matrices (composts, waters...)
- Validation of qPCR system according to french national norm XPT90E
- Technical transfer of developped analysis to routine laboratory (and technical support)
- Member of technical french national committee XPT90E


2004-2005

Bio-Rad, Marnes-la-coquette, France

PCR Project manager (« Food Science » division) :

- Detection kits development for Legionella spp and Legionella pneumophila (quantitative and qualitative) by qPCR (molecular beacon probes)
- Management of a R&D team
- Technical transfer to production and procedures
- Technical assessment of genomic DNA extraction with grafted magnetic beads


2001- 2002
National Institute of Agronomic Research - Jouy-en-Josas - France

Engineer :

- Technical development of methods for research of transgenic corn DNA and its protein called CryIAb from the transgene in blood and milk (ELISA and qPCR techniques)
- Publication in JAOAC (Petit et al, 2005)

Schools attended

DESS ABC (DESS Analyses biologiques et chimiques)
Université de Sussex (School of Biological Sciences)

Since 2005: Veolia Environment

Project assistant
In charge of all the qPCR development in the microbiology R&D central laboratory of Veolia.
I work on the development of detection method by qPCR on bacteria, viruses, fungi and others environment microorganisms being pathogen for humans.
Then I do validation of these methods in order to transfer them to the routine lab.
I also evaluate nucleic acid extraction method.
Sector: Water - Electricity - Gas

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Interests

Competition sport (Ultimate frisbee), outdoor, mountain.

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