Nazaré PEREIRA RODRIGUES

Projects and achievements:

- Development of microfluidic biochips for real-time analysis of cell activity
-- Fabrication of a cell-based biochip with integrated electrochemical sensors
-- Integration of amperometric glucose and oxygen sensors in a microfluidic biochip
-- Electrochemical multidetection of glucose and oxygen in the dynamic conditions of liquid flow

- Artificial micro-environments for cell-culture
-- Cell morphology analysis by fluorescence imaging : comparison of different materials of cell chambers
-- Real-time oxygen monitoring during cell culture : comparison of cell metabolism

- Diabete monitoring
-- Development of ChemFET for the on-line monitoring of calcium produced by beta-cells

Sector: Research and development

Project:

Development of electrochemical devices/sensors for in-situ monitoring and activation of free radicals

Main Achievements:

- Integrated electrochemical sensors for in-vitro free radicals detection (European Program Cellsens )

-- Development of an integrated in vitro optico-electrochemical sensor system: simultaneous monitoring of intra- and extracellular free radicals
-- Electrochemical multidetection of free radicals released by cells (needle-type electrodes, arrays of thin-film microelectrodes)
-- In vivo experiments: tests on mice.

Extended training and visits: Prof. C. Mc Neil (University of Newcastle, UK, 1 month), Prof. M. Deutsch (Bar-Ilan University, Israel, 1 month) and Prof. W. Schuhmann (Ruhr-Universität, Germany, 1 month).

- Electroactivation and detection of thiols and nitrosothiols
-- Development of new electrode materials
-- Kinetic studies

Extended training and visits: Prof. T. Nyokong (Rhodes University, South Africa, 2 months), Prof. J.H. Zagal (Universidad de Santiago de Chile, 2 months).

Sector: Teaching - Research